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ABT-100 | Inhibitor of FNTB
RATINGS:
Cellular Use: (2 reviews)

In Model Organisms: (2 reviews)
Probe Summary
Selectivity
Potency
In Vivo
Control Compounds
Chemical Information
References
Vendors
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Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
FNTB
  • IC50:0.05 nM
  • EC50:0.73 nM
  • EC50:0.43 nM
Inhibitor
10-1000 nM

Selectivity

In Vitro Selectivity Assessment
Selectivity Assessment Description:
>100 000-fold selective for FNTB over the closely related enzyme PGGT1B (GGTase I)
In Cell Selectivity Assessment
Selectivity Assessment Description:
No activity in panel of 75 off-targets @10 µM; closest hit: phencyclidine PCP receptor IC50 = 3 μ ...

Potency
Cellular
In Vitro

FNTB

Mode of Action: Inhibitor

Structure-Activity-Relationship data available? No

DOI Reference: 10.1097/00001813-200511000-00004

In Vivo Validations

Rat
Dose: 5 mg/Kg
Route of delivery: Intravenous
Plasma half life: 2.3 h
Systemic clearance: 1.2 L/h/Kg
Area Under the Curve:: 3.01 ug*h/mL
Volume of Distribution at Steady-State: 5.6 L/Kg

DOI Reference: 10.1097/00001813-200511000-00004

Dose: 5 mg/Kg
Route of delivery: Oral
Cmax: 0.08 ug/mL
Area Under the Curve:: 0.45 ug*h/mL
Bioavailability: 15.1%

DOI Reference: 10.1097/00001813-200511000-00004

Dose: 25 mg/Kg
Route of delivery: Oral
Cmax: 0.74 ug/mL
Area Under the Curve:: 4.62 uh*h/mL
Bioavailability: 30.7%

DOI Reference: 10.1097/00001813-200511000-00004

Dog
Dose: 2.5 mg/Kg
Route of delivery: Intravenous
Plasma half life: 6.8 h
Systemic clearance: 0.34 L/h/Kg
Area Under the Curve:: 7.7 ug*h/mL
Volume of Distribution at Steady-State: 3.3 L/Kg

DOI Reference: 10.1097/00001813-200511000-00004

Dose: 2.5 mg/Kg
Route of delivery: Oral
Plasma half life: 5.9 h
Cmax: 0.28 ug/mL
Area Under the Curve:: 2.84 ug*h/mL
Bioavailability: 35.1%

DOI Reference: 10.1097/00001813-200511000-00004

Negative Control Compounds

A-108
Notes: A-108 (A-409108): Bovine FNTB: IC50 >100 nM; Shows activity on SIGMAR1 (pKi = 7.04) in the GPCR scan.

Chemical Information

Molecular Formula C27H19F3N4O3
SMILEs Cn1cncc1[C@](O)(COc1ccc(C#N)cc1-c1ccc(OC(F)(F)F)cc1)c1ccc(C#N)cc1
InChI InChI=1S/C27H19F3N4O3/c1-34-17-33-15-25(34)26(35,21-7-2-18(13-31)3-8-21)16-36-24-11-4-19(14-32)12-23(24)20-5-9-22(10-6-20)37-27(28,29)30/h2-12,15,17,35H,16H2,1H3/t26-/m0/s1
Molecular weight 504.14 Da
AlogP 5.043960000000004
HBond acceptors 7
HBond donors 1
Atoms 56

Vendors

Note: This is not an exhaustive list and does not indicate endorsement by the Portal.

Expert Reviews

by SERP
(on 4 Jun 2020)
Cellular Use Rating
In Model Organisms
ABT-100 has been investigated as a novel farnesyltransferase inhibitor in vitro and in vivo. ABT-100 is a highly potent (IC50 = 0.05 nmol/L) inhibitor of the farnesyltransferase and displays >100,000-fold...
by SERP
(on 24 Nov 2020)
Cellular Use Rating
In Model Organisms
ABT-100 appears to be a potent and selective probe compound for the investigation of FNTB biology. Selectivity against the closest related target appears good, though there are little data in the...

Portal Comments

In a 2023 study, Hu et al. evaluated ABT-100 in live-cell assays for Phospholipidosis induction, cautioning about adverse effects at concentrations near 1 uM. (DOI: 10.1016/j.chembiol.2023.09.003)

(last updated: 7 Nov 2023)

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