GNE-886

GNE-886 : Inhibitor of CECR2

Structure

SERP Rating

In Cells

(2 ratings)

In Model Organisms

(0 ratings)

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Probe GNE-886 is in the process of SERP review.

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Information

CECR2

Inhibitor

up to 1 uM

Probe Availability:

MCULE

In Vitro Validations

Uniprot ID: Q9BXF3

Target Class: Epigenetic

Target SubClass: Bromodomain

Potency: IC50

Potency Value: 16 nM

Potency Assay: TR-FRET

PDB ID for probe-target interaction (3D structure): --

Target aliases:

Chromatin remodeling regulator CECR2, KIAA1740, CE ...

DOI Reference: 10.1021/acsmedchemlett.7b00132

Uniprot ID: Q9BXF3

Target Class: Epigenetic

Target SubClass: Bromodomain

Potency: Kd

Potency Value: 42 nM

Potency Assay: BromoSCAN

PDB ID for probe-target interaction (3D structure): --

Target aliases:

Chromatin remodeling regulator CECR2, KIAA1740, CE ...

DOI Reference: 10.1021/acsmedchemlett.7b00132

In Cell Validations

In Vivo Data

No in Vivo Validations

Off-Target Selectivity Assesments

Potency assay (off target): Within target family: GNE-886 has a wide selectivity over the 40 bromodomains tested, with only BRD9 (KD = 2000 μM) and its homologue BRD7 (KD = 1100 μM), as well as TAF1(2) (KD = 0.62 μM) and its homologue TAF1L(2) (KD = 2400 μM), showing significant binding. Outside target family: Screened against a panel of 35 diverse kinases, with no kinase inhibited over 20% at 1 μM.

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SERP ratings and comments

SERP Ratings

In Cell Rating

SERP Comments:

This is a high quality chemical probe for the bromodomain of "Cat Eye Syndrome Chromosome Region Candidate 2" (CECR2). The selectivity of the probe has been comprehensively tested against the bromodomain family (40 members screened) as well as other potential off targets (protein kinases). The few detected off-targets are only weakly inhibited making GNE-886 suitable as an excellent probe for CECR2. Importantly, no activity has been detected for BET family bromodomains. Unfortunately, no negative control compound is available for GNE-886. I recommend therefore using in cellular assays also an orthogonal chemical probe such as NVS-CECR2-1.

(last updated: 5 Feb 2023 )

SERP+ Ratings

In Cell Rating

SERP+ Comments:

GNE-886 was tested against 40 bromodomain proteins and showed generally good selectivity. TAF1 (15 fold) and BRD7 (26 fold) are closest off-targets. In an assay of 35 kinases at 1 µM concentration, Lck was inhibited by <20%. No cytotoxicity assay was performed and the probe is also lacking a negative control. For cellular experiments, concentrations higher than 2.5 µM didn't show any improvement.

(last updated: 22 Mar 2023 )