Chemical Probes logo

NK7-902

NK7-902 : Molecular Glue of NEK7

Structure

Information

  • NEK7
  • Molecular Glue
  • 1 µM

In Vitro Validations

Uniprot ID: Q8TDX7
Target Class: Kinase
Target SubClass: Ser/Thr Kinase
Potency: Kd
Potency Value: 1160 nM
Potency Assay: SPR of ternary complex formation
PDB ID for probe-target interaction (3D structure): 9H59
Target aliases:
Serine/threonine-protein kinase Nek7, NEK7, NEK7_H ...

DOI Reference: 10.1016/j.chembiol.2025.06.005

Uniprot ID: Q8TDX7
Target Class: Kinase
Target SubClass: Ser/Thr Kinase
Potency: Kd
Potency Value: 49 nM
Potency Assay: SPR of binary complex
PDB ID for probe-target interaction (3D structure): --
Target aliases:
Serine/threonine-protein kinase Nek7, NEK7, NEK7_H ...

DOI Reference: 10.1016/j.chembiol.2025.06.005

In Cell Validations

In Vivo Data

Off-Target Selectivity Assesments

Potency assay, off target (cells): Selectivity of NK7-902 was assessed by mass spectrometry-based proteomics analysis of human primary monocytes following an 18 h treatment with 1 μM. NEK7 was the most significantly down-regulated protein across all quantified proteins while NEK6, the protein most homologous to NEK7 (86% identical in their catalytic domain) does not contain a β-hairpin and was not degraded by NK7-902.
Potency assay, off target (cells): Expression proteomics analysis of NK7-902 was also done in human iPS cells which express Sal-like protein 4 (SALL4). NEK7 was the most strongly and significantly decreased protein in iPS cells and SALL4 protein levels were detected but unchanged after treatment with NK7-902. A significant down-regulation of SALL3 , another member of the SALL gene family was observed. FLT3 interacting zinc finger 1 (FIZ1) and the zinc finger transcription factor IKZF4 were also significantly down-regulated by NK7-902 in human iPS cells.
I have extra information to add

SERP ratings and comments

No SERP comments found for NK7-902