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PF-4800567 | PF-4800567 : ATP competitive inhibitor of CSNK1E
RATINGS:
Cellular Use: (3 reviews)

In Model Organisms: (2 reviews)
Probe Summary
Selectivity
Potency
In Vivo
Control Compounds
Chemical Information
References
Vendors
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Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
CSNK1E
  • IC50:32 nM
  • IC50:130 nM
ATP competitive
--

Selectivity

In Vitro Selectivity Assessment

Potency: IC50 - CSNK1D 711 nM

Potency Assay Off-Target:
In vitro kinase assay; 50 additional kinases were tested at 1 and 1 uM. PF-4800567 inhibited 69% of ...
In Cell Selectivity Assessment
Potency Assay Off-Target:
Selective in cell over CSNK1D IC50 = 2.65 uM

Potency
Cellular
In Vitro

CSNK1E

Mode of Action: ATP competitive

Structure-Activity-Relationship data available? Yes

DOI Reference: 10.1124/jpet.109.151415

In Vivo Validations

Mouse
Dose: 100 mg/kg
Route of delivery: Subcutaneous
Cmax: 5250 ng/ml Plasma, 9250 ng/ml Brain
Tmax: 0.5 h Plasma, 0.5 h Brain
Area Under the Curve:: 6030 ng*h/ml Plasma, 11,400 ng*h/ml Brain
Fb : 0.931 Plasma, 0.954 Brain
Organ of interest (O): Brain

DOI Reference: 10.1124/jpet.109.151415

Chemical Information

Molecular Formula C17H18ClN5O2
SMILEs Nc1ncnc2c1c(COc1cccc(Cl)c1)nn2C1CCOCC1
InChI InChI=1S/C17H18ClN5O2/c18-11-2-1-3-13(8-11)25-9-14-15-16(19)20-10-21-17(15)23(22-14)12-4-6-24-7-5-12/h1-3,8,10,12H,4-7,9H2,(H2,19,20,21)
Molecular weight 359.11 Da
AlogP 2.992400000000001
HBond acceptors 7
HBond donors 2
Atoms 43

References

Publications

Cross References

ChEMBL BindingDB PDB PubChem ChEBI canSAR

Vendors

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Expert Reviews

by SERP
(on 18 May 2016)
Cellular Use Rating
In Model Organisms
The probe has cellular activity at 2.7 uM, but a target potency of triple-digit nM. Selectivity is ~ 20X at the biochemical level and ~ 10X at the cellular level for CK1d/e. No cellular target-engagement...
by SERP
(on 31 May 2016)
Cellular Use Rating
In Model Organisms
This probe is weakly active in cells (2.7 uM), and mice dosed subcutaneously show only minor effects due to treatment. This probe needs improvement before using in cellular or in vivo models.
by SERP
(on 15 Jan 2017)
Cellular Use Rating
This compound also significantly inhibits EGFR.
Note: The Chemical Probes Portal only endorses compounds as chemical probes for use as specific and selective modulators of the proposed target if they receive three or more (3-4) stars. Read more about our evaluation criteria