USP30inh

USP30inh : Irreversible Inhibitor of USP30

Structure

SERP Rating

In Cells

(1 ratings)

In Model Organisms

(0 ratings)

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Probe USP30inh is in the process of SERP review.

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Information

USP30

irreversible

up to 1 uM
Reviewer recommended concentration: 200 nM

In Vitro Validations

Uniprot ID: Q70CQ3

Target Class: Enzyme

Target SubClass: Peptidase

Potency: IC50

Potency Value: 2.5 nM

Potency Assay: Enzymatic deubiquitination assay

PDB ID for probe-target interaction (3D structure): --

Target aliases:

Ubiquitin carboxyl-terminal hydrolase 30, USP30, U ...

DOI Reference: 10.1016/j.mcpro.2023.100609

In Cell Validations

In Vivo Data

No in Vivo Validations

Off-Target Selectivity Assesments

Potency assay, off target (cells): The endogenous cellular selectivity of USP30inh for USP30 inhibition was confirmed using activity-based protein profiling mass spectrometry (ABPP-MS) against a panel of 49 other endogenous DUBs in neuronal SH-SY5Y cells. Tested up to 25 uM, USP30inh was found to be highly selective for USP30 as it had no significant activity against any of the other 49 endogenous DUBs detected.

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SERP ratings and comments

SERP Ratings

In Cell Rating

SERP Comments:

This is a highly potent USP30 inhibitor that has been screened across a wide panel of deubiquitinases and found to be highly selective within this family. Furthermore, it seems to be able to phenocopy USP30 knock-out (KO) at nanomolar concentrations, without causing additional effects on USP30 KO cells. Further investigation might be needed to confirm selectivity proteome-wide in cells. The tight binding mode of action make this act as an almost irreversible inhibitor, albeit not apparently acting via covalent binding.

(last updated: 26 Jun 2025 )